LIMK1 polyclonal antibody
产品名称： LIMK1 polyclonal antibody
英文名称： LIMK1 polyclonal antibody
产品编号： PAB3484
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of LIMK1.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human LIMK1.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Rho-associated kinase ROCK activates LIM-kinase 1 by phosphorylation at threonine 508 within the activation loop.
Ohashi K, Nagata K, Maekawa M, Ishizaki T, Narumiya S, Mizuno K.J Biol Chem. 2000 Feb 4;275(5):3577-82.
- 2.
- Signaling from Rho to the actin cytoskeleton through protein kinases ROCK and LIM-kinase.
Maekawa M, Ishizaki T, Boku S, Watanabe N, Fujita A, Iwamatsu A, Obinata T, Ohashi K, Mizuno K, Narumiya S.Science. 1999 Aug 6;285(5429):895-8.
- 3.
- Structural features of LIM kinase that control effects on the actin cytoskeleton.
Edwards DC, Gill GN.J Biol Chem. 1999 Apr 16;274(16):11352-61.
- Applications
- Western Blot (Cell lysate)
- The LIMK1 polyclonal antibody (Cat # PAB3484) is used in Western blot to detect LIMK1 in A-375 cell lysate.
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with LIMK1 polyclonal antibody (Cat # PAB3484) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Entrez GeneID:
- 3984
- Protein Accession#:
- P53667
- Gene Name:
- LIMK1
- Gene Alias:
- LIMK
- Gene Description:
- LIM domain kinase 1
- Omim ID:
- 601329
- Gene Ontology:
- Hyperlink
- Gene Summary:
- There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is likely to be a component of an intracellular signaling pathway and may be involved in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. [provided by RefSeq
- Other Designations:
- LIM motif-containing protein kinase,OTTHUMP00000025066